The primary role of apurinic/apyrimidinic (ap) endonuclease ape1 in human cells is the cleavage of the sugar phosphate backbone 5′ to an ap site in dna to produce a single. Here, we investigate ape1 cleavage of abasic substrates that mimic ape1 interactions at stalled replication forks or gaps. We determine that ape1 has robust activity on.
WTH Meaning » Slang term definition & example usage »
The predominant enzyme in humans responsible for repairing ap lesions is ap endonuclease 1 (ape1). For m ultiple turnover kinetics, dna isin excess ofape1at final concentrations of100and30nm,respectively.forsingle turnover kinetics,ape1 is in excess of dna at finalcon. Besides being a powerful ap endonuclease, ape1 possesses.
